CDK Antibody Sampler Kit

• 产品编号：CST-9868S      品牌：Cell Signaling Technology       原厂货号：9868S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

CDK Antibody Sampler Kit中的每个抗体都能检测其内源性蛋白，且不与家族其他成员发生交叉反应。多克隆抗体是由合成的人源的针对cdc2蛋白酪氨酸（15位）的磷酸化肽段免疫动物采用蛋白A和多肽亲和层析技术纯化生产的。单克隆抗体是由合成的人源的针对CDK2蛋白氨基酸残基、重组人CDK7蛋白、重组人CDK4蛋白氨基酸20-290位、人CDK6蛋白30-65位氨基酸肽段及人CDK9蛋白羧基末端肽段免疫动物生产的。CDK Antbody Sampler Kit能够经济地评价Cdk蛋白。该试剂盒内含足够4次western blot实验的一抗和二抗。周期素依赖性蛋白激酶(CDKs)是细胞周期进程的核心效应器。CDK的活性通过与其细胞周期蛋白伴侣和周期蛋白依赖性激酶抑制剂(CKIs)相互作用而被调节，也可通过激活和抑制磷酸化事件。抑制效应由能够靶向CDK1氨基末端残基的Wee1和Myt1介导(1,2)。Cdc25磷酸酶去磷酸化这些残基将导致CDK激酶活性的激活(3)。CDK7/cyclinH络合物是普遍存在的哺乳动物CDK活化激酶(CAK)，能够磷酸化CDKs中T-回路结构域的保守苏氨酸残基。 RNA聚合酶II的羧基端结构域也是CAK以及CDK9/cyclinT的靶标(4,5)。CDK4/ 6联和Cyclin D并磷酸化视网膜母细胞瘤蛋白，通过G1期限制点启动周期进展(6)。CDK2能够联合早S期的cyclinE和G2后期的cyclinA。CDK1/cyclinB调节有丝分裂事件的启动(7)。

原厂资料：

Specificity / Sensitivity

Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr15 of human cdc2; polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, amino acids 270-290 of recombinant human CDK4, amino acids 30-65 of human CDK6 and residues near the carboxy terminus of human CDK9.

Description

The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.

Background

Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7). 

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.