Protein A Agarose Beads

产品编号：CST-9863P 品牌：CST 原厂货号：9863P
产品分类：生化和细胞分析 > 微珠和磁珠 > 配体耦联磁珠 > Protein A&G 磁珠
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运保温度:

4ºC.

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描述：

蛋白A琼脂糖微球是一种亲和力矩阵用于从免疫沉淀(IP)中分离小规模的免疫复合物。蛋白A与琼脂糖共价偶联，表现出与很多物种（包括人类、兔子、小鼠、大鼠和养）的IgG亚型的高亲和力，并且可以与这些抗体共同应用于免疫沉淀实验。短暂涡旋管子以重悬微球，每一个免疫沉淀反应加入20-40ul微球。对于清洗微球和随后的洗脱免疫复合物，微球可以通过在微型离心机以6000rpm离心1分钟从溶液中分离。采用涡旋或者摇摆管子的方法用溶液重悬微珠。请按照赛信通公司建议的免疫沉淀（IP）步骤进行IP，随后进行western blot实验。

原厂资料：

Description

Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Directions for Use

Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

注意事项：

Storage: Supplied as a 50% slurry in 18.5% ethanol solution.
Store at 4º