Bisindolylmaleimide I Hydrochloride

• 产品编号：CST-9841S      品牌：CST       原厂货号：9841S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

Bisindolylmaleimide I 以500ug粉剂提供。储存在-20℃。使用前，将粉末溶解于0.28ml DMSO中产生4mM原液。对于工作浓度为2uM-4uM，将原液按1:2000到1:1000稀释。使用需要的浓度处理细胞30分钟。Bisindolylmaleimide I在粉剂状态比溶液中更稳定；因此推荐在使用前再将其溶解。储存在-20℃或更低温度。Bisindolylmaleimide I(BIS)是PKC的强力抑制剂（1,2）。BIS在体外试验的IC50，对PKCα/β/γ是10-20nM，对PKCδ/ε是100-200nM。在体外对PKCζ的IC50是6uM，表明BIS是此亚型非常弱的抑制剂。体内细胞分析BIS对PKC的IC50在0.2-2uM（1,3）。

1. Toullec, D. et al. (1991) J. Biol. Chem. 266, 15771-15781.
2. Martiny-Baron, G. et al. (1993) J. Biol. Chem. 268, 9194-9197.
3. Heikkilä, J. et al. (1993) Biochem. Biophys. Res. Commun. 197, 1185-1193.

原厂资料：

Directions for Use

Bisindolylmaleimide I is supplied as 500 μg powder. Store at or below -20ºC. Before use, dissolve powder in 0.28 ml DMSO to make a 4 mM bisindolylmaleimide I stock solution. For working concentrations of 2 μM-4 μM, dilute DMSO stock 1:2000 to 1:1000. Treat cells with the desired concentration for 30 minutes. Bisindolylmaleimide I is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Background

Bisindolylmaleimide I (BIS) is a potent inhibitor of PKC (1,2). In vitro the IC50 of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC50 for PKCζ is about 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays the IC50 of BIS for PKC is between 0.2-2 μM (1,3).

1. Toullec, D. et al. (1991) J. Biol. Chem. 266, 15771-15781.
2. Martiny-Baron, G. et al. (1993) J. Biol. Chem. 268, 9194-9197.
3. Heikkilä, J. et al. (1993) Biochem. Biophys. Res. Commun. 197, 1185-1193.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw