SignalSilence? hnRNP LL siRNA I (Mouse Specific)

• 产品编号：CST-9825S      品牌：CST       原厂货号：9825S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® hnRNP LL siRNA I (Mouse Specific)抑制小鼠和大鼠hnRNP LL蛋白表达。Cell Signaling Technology (CST)公司的SignalSilence® hnRNP LL siRNA I (Mouse Specific)允许研究者通过RNA干扰去特异性抑制hnRNP LL蛋白表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST公司推荐在细胞裂解前48到72小时转染100 nM SignalSilence® hnRNP LL siRNA I (Mouse Specific)。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL)是一个细胞核RNA结合蛋白，它与hnRNP L蛋白共有69% 氨基酸同源序列，hnRNP L是hnRNP复合物的组分，能够调控mRNA生成，包装以及加工(1)。 hnRNP LL蛋白在活化的T细胞中被诱导，并且可作为对T细胞发育和激活至关重要的酪氨酸磷酸酶CD45选择性剪接的关键调控因子(2)。hnRNP LL 还能够调控CD44 和Stat5a的选择性剪接(2)。hnRNP LL有四个异构体，都是通过选择性剪接产生的，并且在人类组织中广泛表达(3)。

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

SignalSilence® hnRNP LL siRNA I (Mouse Specific) inhibits mouse and rat hnRNP LL expression.

Description

SignalSilence® hnRNP LL siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP LL expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® hnRNP LL siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNP L, a component of hnRNP complex that regulates mRNA formation, packaging, and processing (1). hnRNP LL is induced in activated T cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T cell development and activation (2). hnRNP LL also regulates the splicing of CD44 and Stat5a (2). The four isoforms of hnRNP LL are generated by alternative splicing and are widely expressed in human tissues (3).

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

注意事项：

Storage: hnRNP LL siRNA I (Mouse Specific) is supplied in
RNAse-free water. Aliquot and store at -20ºC.