Mismatch Repair Antibody Sampler Kit

• 产品编号：CST-9786S      品牌：Cell Signaling Technology       原厂货号：9786S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

单克隆抗体是由截短的重组MBP-MLH1或由合成的人源的针对MSH2蛋白氨基末端的肽段免疫动物生产的。多克隆抗体是由合成的人源的针对MLH6蛋白羧基末端的肽段免疫动物生产的。所有抗体均采用蛋白A和多肽亲和层析技术纯化生产。Mismatch Repair Antibody Sampler Kit为研究细胞内DNA错配修复系统提供了一个经济的方式。该试剂盒提供了足够4次western blot实验的一抗和二抗。DNA错配修复系统(MMR)能够修复后复制DNA，抑制不相同的DNA序列之间的重组，并诱导伴随某些类型DNA损伤的检验点和凋亡反应(1)。MSH2(MutS蛋白同源物2) 与MSH6形成hMutS-α二聚体而且是错配修复过程中的一个主要组成部分。 hMutS-α是BRCA1基因相关监视复合物(BASC)的一部分，该复合物还包含BRCA1，MLH1，ATM，BLM，PMS2蛋白和Rad50-Mre11-NBS1 复合物(2)。一大部分遗传性非息肉病性结直肠癌(Lynch综合征)中已发现MSH6和其它MMR蛋白的突变，该病西方世界最常见的一种遗传性大肠癌(3)。已有显示MSH6突变发生在胶质母细胞瘤以响应替莫唑胺治疗，并可促进替莫唑胺抵抗(4)。

1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
2. Wang, Y. et al. (2000) Genes Dev 14, 927-39.
3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83.
4. Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.

原厂资料：

Specificity / Sensitivity

MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein. MSH2 (D24B5) XP® Rabbit mAb detects endogenous levels of total MSH2 protein. MSH6 (L990) Antibody detects endogenous levels of total MSH6 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with truncated recombinant MBP-MLH1 or by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MSH2. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLH6 protein. Antibodies are purified using protein A and peptide affinity chromatography.

Description

Mismatch Repair Antibody Sampler Kit provides an economical means to investigate the DNA mismatch repair system within the cell. The kit contains primary and secondary antibodies to perform four western blots with each antibody.

Background

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
2. Wang, Y. et al. (2000) Genes Dev 14, 927-39.
3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83.
4. Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.