Neurofilament Antibody Sampler Kit

• 产品编号：CST-9781S      品牌：Cell Signaling Technology       原厂货号：9781S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Neurofilament-L(DA2)Mouse mAb 鼠单抗和Neurofilament-L (C28E10)Rabbit mAb 兔单抗识别内源性的Neurofilament-L总蛋白。Neurofilament-M (RMO 14.9)Mouse mAb 鼠单抗识别内源性的Neurofilament-M总蛋白。Neurofilament-H (RMdO 20)Mouse mAb 鼠单抗识别内源性的Neurofilament-H总蛋白。Neurofilament-H (RMdO 20) Mouse mAb 鼠单抗被报道能够识别人类样本中的NFM和NFH，但只能识别小鼠、大鼠及牛样本中的NFH (Lee, V.M. et al., 1988)。该单克隆抗体由纯化和酶去磷酸化的猪neurofilament（神经丝蛋白）、轻链（Neurofilament-L (DA2)）、合成的人Neurofilament-L (Neurofilament-L (C28E10)) Glu450位点附近肽段、大鼠neurofilament (Neurofilament-H (RMdO 20))免疫动物而制备的。#2836神经丝蛋白中链(Neurofilament-M (RMO 14.9))或大鼠神经丝蛋白(Neurofilament-H (RMdO 20))免疫动物而制。#2836单克隆抗体由大鼠neurofilament免疫动物而制。#2835单克隆抗体由纯化和酶去磷酸化的猪neurofilament轻链免疫动物而制。#2837单克隆抗体由合成的人Neurofilament-L (Neurofilament-L (C28E10)) Glu450位点附近肽段免疫动物而制。#2838单克隆抗体由大鼠neurofilament中链免疫动物而制。Neurofilament Antibody Sampler Kit 小包装抗体试剂盒提供了一个经济的手段来研究Neurofilament。该试剂盒包含了足够的一抗和二抗以满足每款抗体用于4张膜的免疫印迹实验。细胞骨架由三种胞内纤维构成：微丝、中间纤维和微管。Neurofilament是神经元中发现的主要的中间纤维，是组成轻 (NFL)、中 (NFM)、重 (NFH)三种纤维的亚基。与其它中间纤维蛋白的结构类似，neurofilament 也具有球状的氨基端头，中央α-螺旋杆结构域和一个羧基端尾。它的前体纤维由一种异型四聚体单位（NFL-NFM和NFL-NFH）构成，八条这样的前体纤维组成一个典型的10nm的中间纤维。Neurofilament在轴突径向生长和确定轴突口径中起作用，微管参与轴突伸长。PKA磷酸化NFL和NFM的头端结构域，抑制neurofilament聚集（3,4）。Neurofilament的聚集在许多人类神经系统疾病中被发现，包括帕金森病（与Lewy小体中α-synuclein伴随）、阿尔茨海默氏病、腓骨肌萎缩症、肌萎缩性侧索硬化症（ALS）（1）。

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

原厂资料：

Specificity / Sensitivity

Neurofilament-L (DA2) Mouse mAb and Neurofilament-L (C28E10) Rabbit mAb detect endogenous levels of total Neurofilament-L protein. Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988).

Source / Purification

Monoclonal antibodies are produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain (Neurofilament-L (DA2)), a synthetic peptide surrounding Glu450 of human Neurofilament-L (Neurofilament-L (C28E10)), rat neurofilament medium chain(Neurofilament-M (RMO 14.9)), or rat neurofilament (Neurofilament-H (RMdO 20)).#2836 Monoclonal antibody is produced by immunizing animals with rat neurofilament.#2835 Monoclonal antibody is produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain.#2837 Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.#2838 Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.

Description

The Neurofilament Antibody Sampler Kit provides an economical means of evaluating neurofilaments. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.