Loading Control Antibody Sampler Kit (Mouse)

产品编号：CST-9774S 品牌：Cell Signaling Technology 原厂货号：9774S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C.

到货周期：

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其它组分：

Histone H3 (96C10) Mouse mAb 【子货号：#3638，包装：40 µl，，运保温度：–20°C.】

β-Actin (8H10D10) Mouse mAb 【子货号：#3700，包装：40 µl，，运保温度：–20°C.】

α-Tubulin (DM1A) Mouse mAb 【子货号：#3873，包装：40 µl，，运保温度：–20°C.】

Anti-mouse IgG, HRP-linked Antibody 【子货号：#7076，包装：100 µl，，运保温度：–20°C.】

描述：

小包装抗体套装中的每一个抗体可以检测出内源性目的蛋白，而通常不会同其它蛋白发生交叉反应。单克隆抗体是通过采用分别与人类β-actin氨基末端、组蛋白H3羧基末端相对应的残基合成的肽段或者采用鸡大脑中提取的全长α-tubulin来免疫动物生产的。鼠源α-tubulin表位单克隆抗体可以识别人类α-tubulin蛋白Val440 周围残留蛋白。管家蛋白在细胞中执行多种功能，连续高表达在多种组织和细胞中。Western blot 方法一般采用管家蛋白如β-actin, 组蛋白 H3, and α-tubulin作为上样对照。Actin是一种泛素化蛋白，是真核细胞骨架的重要组成成分，通常以F-actin纤维多聚体的形式存在（1）。球形微球蛋白亚基由α- and β-tubulin组成，是微管蛋白的构成成分。而后者是构成细胞骨架的三种细胞质纤维蛋白之一（2）。组蛋白蛋白质包括组蛋白H3，组成染色质的初级结构，也称为核小体。染色质结构的突变对调节真核细胞的转录起着非常重要的作用（3）

原厂资料：

Specificity / Sensitivity

Each antibody in the Loading Control Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross-react with other proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human β-actin, the carboxy terminus of human histone H3, or with full-length chicken α-tubulin purfied from brain extracts. The epitope of α-Tubulin (DM1A) Mouse mAb recognizes residues surrounding Val440 of human α-tubulin protein.

Description

The Loading Control Antibody Sampler Kit provides an economical means to detect a variety of housekeeping proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.

Background

Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, histone H3, and α-tubulin as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (2). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (3).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.