Caspase-6 Antibody

• 产品编号：CST-9762S      品牌：CST       原厂货号：9762S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源性的全长的caspase-6 (35 kDa)和其在193位天门冬氨酸处裂解产生的小亚基 (15 kDa）。该抗体不能识别其它caspases。该多克隆抗体采用合成的caspase-6裂解位点的氨基酸序列相应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。Caspase-6 (Mch2) 是一种在细胞凋亡过程中发挥主要作用的效应酶（1.2）。一旦接收凋亡信号刺激，caspases启动酶如caspase-9 等被裂解而活化（3）。上游活化的caspases 进一步裂解下游caspases效应酶如caspase-3 和caspase-6, 产生大小亚单位，使之活化，进而启动caspase级联反应导致凋亡（4.5）。 膜相关蛋白lamin A 是caspase-6的主要靶蛋白之一（6）。caspase-6的裂解导致细胞膜功能障碍，形成膜泡，最终导致细胞死亡。

1. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
2. Faleiro, L. et al. (1997) EMBO J 16, 2271-81.
3. Li, P. et al. (1997) Cell 91, 479-89.
4. Slee, E.A. et al. (1999) J Cell Biol 144, 281-92.
5. MacFarlane, M. et al. (1997) J Cell Biol 137, 469-79.
6. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.

原厂资料：

Specificity / Sensitivity

Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35 kDa) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15 kDa). This antibody does not recognize other caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.

1. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
2. Faleiro, L. et al. (1997) EMBO J 16, 2271-81.
3. Li, P. et al. (1997) Cell 91, 479-89.
4. Slee, E.A. et al. (1999) J Cell Biol 144, 281-92.
5. MacFarlane, M. et al. (1997) J Cell Biol 137, 469-79.
6. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.