Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb

• 产品编号：CST-9748S      品牌：CST       原厂货号：9748S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源性水平的Asp384位剪切的caspase-8小片段。其与全长 caspase-8不发生交叉反应。该单克隆抗体是采用合成的人源caspase-8 p10片段的氨基末端周围残基相对应的多肽免疫动物后制备获得。该抗体存在于含50％ 甘油和低于0.02％ 叠氮化钠的HEPES缓冲液中。CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8，并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases，如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化，包括DNA的片段化和细胞固缩。

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb detects endogenous levels of the small fragment of caspase-8 resulting from cleavage at aspartic acid 384. The antibody does not cross-react with full length caspase-8.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of p10 of human caspase-8. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.