MEF2A Antibody

• 产品编号：CST-9736S      品牌：CST       原厂货号：9736S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MEF2A Antibody兔多抗能检测内源性总MEF2A 蛋白水平。该抗体不与相关家族成员发生交叉反应。该多克隆抗体是通过用人源MEF2A蛋白残基的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。MEF2A是转录因子MEF2（肌细胞增强子） 家族的成员，在哺乳动物体内，四个MEF2 A相关基因（MEF2A, MEF2B, MEF2C和 MEF2D）编码的蛋白在它们的DNA结合结构域有显著地氨基酸序列相似性，而其他部分的蛋白相似性较低(1)。起初人们认为MEF2家族成员是肌肉特异性DNA结合蛋白，它能识别许多肌肉特异性基因启动子内的MEF2结构(2,3)。p38 MAP激酶磷酸化MEF2A蛋白转录激活区域的苏氨酸（312、319位点），从而增强了MEF2A-MEF2D异二聚体依赖的基因表达(4)。另一方面，凋亡刺激因子（如神经毒的入侵）导致CDK5依赖型MEF2A蛋白激活区域丝氨酸（408位点）的磷酸化，抑制了MEF2A的促存活效应(5)。

1. Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13.
2. Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656.
3. Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798.
4. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
5. Gong, X. et al. (2003) Neuron 38, 33-46.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

MEF2A Antibody detects endogenous levels of total MEF2A protein. The antibody does not cross-react with related family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human MEF2A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MEF2A is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, four MEF2A-related genes (MEF2A, MEF2B, MEF2C and MEF2D) encode proteins which exhibit significant amino acid sequence similarity within their DNA binding domains and to a lesser extent throughout the remaining proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Phosphorylation of MEF2A at Thr312 and Thr319 within the transcription activation domain by p38 MAP kinase enhances MEF2A-MEF2D heterodimer-dependent gene expression (4). On the other hand, apoptotic stimuli (e.g. neurotoxic insult) result in CDK5-dependent phosphorylation of MEF2A at Ser408 within the activation domain, inhibiting MEF2A pro-survival function (5).

1. Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13.
2. Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656.
3. Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798.
4. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
5. Gong, X. et al. (2003) Neuron 38, 33-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody