SMYD2 (D14H7) Rabbit mAb

• 产品编号：CST-9734S      品牌：CST       原厂货号：9734S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SMYD2 (D14H7) Rabbit mAb兔单抗能够检测内源性SMYD2总蛋白水平。通过合成的与人源SMYD2蛋白Val414位点周围相应的多肽片段去免疫动物从而制备出此单克隆抗体。该抗体不与SMYD蛋白发生交叉反应。SET and MYND domain-containing protein 2 (SMYD2)也称为lysine methyltransferase protein 3C (KMT3C)，它是SMYD蛋白甲基转移酶家族的成员(1)。该家族的五个成员(SMYD1、SMYD2、SMYD3、SMYD4和SMYD5)包含一个保守的催化SET结构域，起初被鉴定在Drosophila Su[var]3-9、Enhancer of zeste和Trithorax蛋白。通过MYN结构域/锌指结构域可以分离该结构域，这被认为有助于蛋白质-蛋白质相互作用(1)。SMYD2定位在细胞质和细胞核内，并且与发育的小鼠胚胎一样，在成年小鼠心脏、大脑、肝脏、肾脏、胸腺和卵巢中高度表达(1)。通过与Sin3A抑制复合物相互作用和使histone H3蛋白Lys36位点甲基化，SMYD2的功能是去抑制转录(1)。SMYD2也与HSP90α相互作用，以及使histone H3蛋白Lys4位点甲基化，而histone H3蛋白是与转录激活相关的标志(2)。除了组蛋白作为甲基化底物，SMYD2可以使p53蛋白在Lys370位点甲基化从而抑制p53介导的转录激活和凋亡(3,4)。

1. Brown, M.A. et al. (2006) Mol Cancer 5, 26.
2. Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
3. Huang, J. et al. (2006) Nature 444, 629-32.
4. Huang, J. et al. (2007) Nature 449, 105-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Horse

Specificity / Sensitivity

SMYD2 (D14H7) Rabbit mAb recognizes endogenous levels of total SMYD2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val414 of human SMYD2 protein. This antibody is not predicted to cross-react with other SMYD proteins.

Background

SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), is a member of the SMYD family of protein methyltransferases (1). All five members of this family (SMYD1, SMYD2, SMYD3, SMYD4, and SMYD5) contain a conserved catalytic SET domain, originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. This domain is split by the MYN domain/zinc finger motif believed to facilitate protein-protein interactions (1). SMYD2 localizes to both the cytoplasm and nucleus, and is highly expressed in the adult mouse heart, brain, liver, kidney, thymus, and ovary, as well as in the developing mouse embryo (1). SMYD2 functions to repress transcription by interacting with the Sin3A repressor complex and methylating Lys36 of histone H3 (1). SMYD2 also interacts with HSP90α and methylates Lys4 of histone H3, a mark associated with transcriptional activation (2). In addition to histones as methyl substrates, SMYD2 methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (3,4).

1. Brown, M.A. et al. (2006) Mol Cancer 5, 26.
2. Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
3. Huang, J. et al. (2006) Nature 444, 629-32.
4. Huang, J. et al. (2007) Nature 449, 105-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.