DFF45/DFF35 Antibody

• 产品编号：CST-9732S      品牌：CST       原厂货号：9732S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DFF45/DFF35 抗体能够检测内源的全长DFF45和（或）DFF35，其与其它相关蛋白不发生交叉反应。该多克隆抗体是采用合成的人源DFF45蛋白Asp224周围氨基酸序列相应的肽段免疫动物后获得。抗体通过蛋白A和肽段亲和层析而纯化。在正常细胞中，人源DFF45和它的鼠种同源物ICAD在caspase 激活的脱氧核糖核酸酶(DFF40 或 CAD)合成过程中，作为他们的伴侣蛋白而发挥功能(1)。DFF45（或其亚型DFF35）与DFF40相互作用，从而抑制后者的DNase的活性(1-4)。在体外，DFF45是多种caspases的靶标，包括caspase-3、-6、-7、-8 和颗粒酶B (3)。在体内，caspase-3被认为是促进DFF45水解并释放羧基端片段这一过程的首要酶(3,5)。DFF45的裂解钝化了它对DFF40的抑制作用，并引起DFF40降解核DNA，进而导致细胞死亡 (6,7)。

1. Enari, M. et al. (1998) Nature 391, 43-50.
2. Sakahira, H. et al. (1998) Nature 391, 96-99.
3. Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
4. Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
5. Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
6. Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
7. Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.

原厂资料：

Specificity / Sensitivity

DFF45/DFF35 Antibody detects endogenous levels of total full length DFF45 and/or full length DFF35. The antibody does not cross-react with related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).

1. Enari, M. et al. (1998) Nature 391, 43-50.
2. Sakahira, H. et al. (1998) Nature 391, 96-99.
3. Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
4. Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
5. Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
6. Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
7. Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.