该产品能检测细胞中外源性表达的DYKDDDDK标记的蛋白。本抗体能识别融合到目标蛋白氨基端或羧基端的DYKDDDDK肽。本抗体的结合专一性并不依赖于二价金属阳离子的存在。多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。该抗体与Alexa Fluor® 594荧光染料结合，并且在转染了DYKDDDDK标记的蛋白的细胞中进行了直接免疫荧光分析的内部测试。本抗体预计有与unconjugated DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368一样的种交叉反应。附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。DYKDDDDK肽作为一个普通的附加表位广泛地应用于表达载体中。此肽能与相关蛋白一起作为氨基端或羧基端融合蛋白进行表达和检测（1）。
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 594 Conjugate) detects exogenously expressed DYKDDDDK-tagged proteins in cells. The antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in cells transfected with DYKDDDDK-tagged protein. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
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