PIP5K1A Antibody

• 产品编号：CST-9693S      品牌：CST       原厂货号：9693S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PIP5K1A抗体识别内源性的全部PIP5K1A蛋白。该多克隆抗体用与人类PIP5K1A蛋白中Gly64位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。磷脂酰肌醇-4-磷酸 5-激酶（PIP5K）合成磷脂酰肌醇-4,5-二磷酸（PtdIns（4,5）P2），一种磷酸肌醇信号的关键前体，也直接调节某些蛋白质和细胞过程。PIP5K有两个亚族，I和II，可以从不同的底物池中产生tdIns(4,5)P2。PIP5K1使用PtdIns4P作为底物，PIP5K2使用PtdIns5P作为底物（1,2）。在哺乳动物细胞中，每个PIP5K1和PIP5K2亚族中还有三种亚型，分别由不同的基因编码，命名为 α,β和γ（3-7），更多的PIPK可能仍然有待被发现。所有PIP5K亚型均被磷脂酸激活，被ARF和Rho GTP酶广泛调节，被蛋白激酶A和PI激活的自身磷酸化所抑制（8）。

1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

原厂资料：

Specificity / Sensitivity

PIP5K1A Antibody detects endogenous levels of total PIP5K1A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Gly64 of human PIP5K1A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphatidylinositol-4-phosphate 5-kinases (PIP5K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that also regulates some proteins and cellular processes directly. There are two subfamilies of PIP5Ks, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP5K1s use PtdIns4P as a substrate, whereas PIP5K2s use PtdIns5P (1,2). In mammalian cells, three isoforms of each PIP5K1 and PIP5K2 subfamily, encoded by distinct genes, have been characterized and named α, β and γ (3-7), and additional PIPKs likely remain to be discovered. All PIP5K isoforms are stimulated by phosphatidic acid, are extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.