Nitro-Tyrosine Antibody

• 产品编号：CST-9691S      品牌：CST       原厂货号：9691S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nitro-Tyrosine Antibody兔多抗可以识别包含硝基酪氨酸的蛋白或肽段，不受周围氨基酸序列影响。这是发现新的硝基蛋白、分析蛋白硝基化和测量组织样本中硝基蛋白含量的有用工具。此抗体不与未修饰的酪氨酸或磷酸化酪氨酸发生交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）。该多克隆抗体用合成的包含硝基化酪氨酸的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化制备。一氧化氮（NO）与肿瘤生成（1）、慢性感染、炎症（2）和神经退行性病变（3）有关。组织中高水平的过氧化物或一氧化氮相互作用形成过氧硝酸盐，它可以将蛋白中的酪氨酸残基转化为3-硝基-酪氨酸（4）。线粒体锰超氧化物歧化酶的酪氨酸硝基化可导致酶活性的丧失（4）。p53酪氨酸残基硝基化会消除其与DNA一致序列的结合能力（5）。

1. Bentz, B.G. et al. (2000) Head Neck 22, 64-70.
2. Jaiswal, M. et al. (2000) Cancer Res 60, 184-90.
3. Olivenza, R. et al. (2000) J Neurochem 74, 785-91.
4. MacMillan-Crow, L.A. et al. (1996) Proc Natl Acad Sci U S A 93, 11853-8.
5. Chazotte-Aubert, L. et al. (2000) Biochem Biophys Res Commun 267, 609-13.

原厂资料：

Specificity / Sensitivity

Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. The antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic nitro-tyrosine-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).

1. Bentz, B.G. et al. (2000) Head Neck 22, 64-70.
2. Jaiswal, M. et al. (2000) Cancer Res 60, 184-90.
3. Olivenza, R. et al. (2000) J Neurochem 74, 785-91.
4. MacMillan-Crow, L.A. et al. (1996) Proc Natl Acad Sci U S A 93, 11853-8.
5. Chazotte-Aubert, L. et al. (2000) Biochem Biophys Res Commun 267, 609-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody