PAX2 Antibody

• 产品编号：CST-9666S      品牌：CST       原厂货号：9666S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PAX2抗体可以识别内源性的总PAX2蛋白。多抗由合成肽段免疫动物产生，该肽段与人PAX2蛋白邻近His282氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Paired box (PAX)蛋白是一组在动物发育过程中发挥了重要的及多样性作用的转录因子家族（1）。人和其他哺乳动物中发现了9个PAX蛋白（PAX1-9）。它们都含有氨基端“配对”结构域，包括了2个具有DNA结合活性的螺旋-转角-螺旋基序（2）。PAX蛋白，基于一个羧基端同源域和中心八肽区域的存在与否可以根据不同的结构分为4个不同的亚型(I-IV)。亚型I (PAX1 和9)包括八肽但是缺乏同源域；亚型II (PAX2, 5, 和 8)包括了八肽和被截短的同源域；亚型III (PAX3 和7)包括了八肽和完整的同源域；和亚型IV (PAX4 和6)包括了一个完整的同源域但是缺乏八肽结构（2）。PAX蛋白在发育过程中发挥了重要作用调控了胚胎模式和器官发生过程中的转录调控网络（3）；PAX蛋白的一组亚型还在产后发育过程中发挥重要功能（4）。研究提示基因突变引发的PAX基因表达异常与多种癌症相关（1-3），部分亚型II和III被认为是癌症进程中可能的介导因子（2）。PAX2涉及到了神经系统和包括肾脏和泌尿生殖道的排泄系统，视柄，耳，脑后脑交界处，以及脊髓的发育过程。

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
5. Schedl, A. and Hastie, N.D. (2000) Curr Opin Genet Dev 10, 543-9.
6. Torres, M. et al. (1995) Development 121, 4057-65.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

PAX2 Antibody recognizes endogenous levels of total PAX2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His282 of human PAX2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).PAX2 is involved in the development of the nervous and excretory systems including the kidney and urogentical tract, the optic stalk, ear, midbrain-hindbrain junction, and the spinal cord (5,6).

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
5. Schedl, A. and Hastie, N.D. (2000) Curr Opin Genet Dev 10, 543-9.
6. Torres, M. et al. (1995) Development 121, 4057-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.