PTEN and PDK1 Antibody Sampler Kit

• 产品编号：CST-9652S      品牌：Cell Signaling Technology       原厂货号：9652S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

PTEN and PDK1 Sampler Kit中的每一个抗体可以检测其内源性的靶蛋白的水平。而激活状态的特异抗体可以检测在特定位点磷酸化的靶蛋白。 Non-Phospho PTEN (Ser380/Thr382/Thr383) Antibody可以检测到在Ser380, Thr382 和 Thr383位点发生磷酸化的内源性PTEN水平。磷酸化特异的兔单克隆抗体是采用与人类PTEN的Ser380, Thr382 和 Thr383，人类 PDK1的Ser241周围残基相对应的合成磷酸肽(KLH-coupled)免疫动物生产的。 PTEN (D4.3) XP™ 单抗是采用人类PTEN羧基末端序列相对应的合成肽段免疫动物生产的。多克隆抗体是采用采用人类PTEN的Ser380, Thr382 和 Thr383以及人类 PDK1的羧基末端残基相对应的合成肽段免疫动物生产的。多克隆抗体是通过蛋白A和肽亲和层析法纯化的。PTEN and PDK1 Sampler Kit为评估调节多信号通路的两个关键的酶提供了经济的检测方法。本试剂盒包含足够量的一抗和二抗来进行四次western blots实验。PTEN（phosphatase and tensin homologue deleted on chromosome ten），也称为MMAC（ (mutated in multiple advanced cancers)磷酸酶，是一种存在于多种人类癌症中的肿瘤抑制因子（1）。PTEN编码一个403个氨基酸组成的多肽，最初被认为是一个双特异性蛋白磷酸酶（2）。PTEN的主要底物是3磷酸肌醇激酶（PI3K）激活转化生成的肌醇磷脂（3）。PTEN是PI3K/Akt信号通路的一个主要的负调因子（1,4,5）。PTEN有一个羧基端的非催化调控的结构域，其中含有3个可以调节PTEN的稳定性，也可能影响其生物活性的磷酸化位点（Ser380，Thr382，Thr383）（6,7）。PTEN调节p53蛋白水平和活性（8），并在趋化过程中参与G蛋白偶联信号转导（9,10）。磷酸肌醇依赖蛋白激酶1（PDK1）在多种信号传导通路中发挥核心作用（1,2），包括Akt和PKC同工酶p70 S6激酶和RSK的活化（3）。通过这些激酶，PDK1参与调控了广泛的生物学过程，包括细胞增殖，分化和凋亡。

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
11. Belham, C. et al. (1999) Curr Biol 9, R93-6.
12. Toker, A. and Newton, A.C. (2000) Cell 103, 185-8.
13. Williams, M.R. et al. (2000) Curr Biol 10, 439-48.

原厂资料：

Specificity / Sensitivity

Each antibody in the PTEN and PDK1 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. Non-Phospho-PTEN (Ser380/Thr382/Thr383) Antibody detects endogenous levels of PTEN only when dephosphorylated at Ser380, Thr382 and Thr383.

Source / Purification

Phospho-specific rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN and around Ser241 of human PDK1. PTEN (D4.3) XP® Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser380/Thr382/Thr383 of human PTEN and surrounding the carboxy terminus of human PDK1. Polyclonal antibodies are purified using protein A and peptide affinity chromatography.

Description

The PTEN and PDK1 Sampler Kit provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit contains enough primary and secondary antibodies to perform four Western blots per primary antibody.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
11. Belham, C. et al. (1999) Curr Biol 9, R93-6.
12. Toker, A. and Newton, A.C. (2000) Cell 103, 185-8.
13. Williams, M.R. et al. (2000) Curr Biol 10, 439-48.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody