PA28α (D1C10) Rabbit mAb

• 产品编号：CST-9643S      品牌：CST       原厂货号：9643S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PA28α识别内源性的PA28α总蛋白。该抗体与PA28β/PSME2或PA28γ/PSME3均无交叉反应。该单克隆抗体经合成与人PA28α蛋白氨基末端邻近氨基酸残基序列一致的肽段，免疫动物产生。20S蛋白酶体是细胞内蛋白降解的主要的蛋白水解酶复合物。PA700, PA28, 和PA200是三种主要蛋白复合物，主要作为20S蛋白酶体的激活剂。PA28有3种进化保守的亚基：PA28α (PSME1), PA28β (PSME2), 和PA28γ (PSME3) (1,2)。PA28α 和PA28β形成了一种heteroheptameric复合物，并通过其起始位点与20S复合物结合而发挥功能。PA28α/β复合物广泛存在于细胞中，并通过促进外源性蛋白的抗原多肽的产生，参与MHC I类抗原提呈(2)。PA28γ以homoheptamer的形式存在，并主要定位于细胞核。PA28γ复合物通过与特殊核靶向蛋白的结合与导向，发挥对20S蛋白酶体进一步的降解作用(3,4)。

1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48.
2. Rechsteiner, M. and Hill, C.P. (2005) Trends Cell Biol. 15, 27-33.
3. Zhou, P. (2006) Cell 124, 256-257.
4. Li, X. et al. (2006) Cell 124, 381-392.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig

Specificity / Sensitivity

PA28α recognizes endogenous levels of total PA28α protein. This antibody does not cross-react with either PA28β/PSME2 or PA28γ/PSME3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PA28α protein.

Background

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4).

1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48.
2. Rechsteiner, M. and Hill, C.P. (2005) Trends Cell Biol. 15, 27-33.
3. Zhou, P. (2006) Cell 124, 256-257.
4. Li, X. et al. (2006) Cell 124, 381-392.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.