Phospho-Tau (Ser396) (PHF13) Mouse mAb

• 产品编号：CST-9632S      品牌：CST       原厂货号：9632S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tau (Ser396) (PHF13) Mouse mAb 鼠单抗可识别内源性的Ser396磷酸化的Tau蛋白。该单克隆抗体用纯化的人类Tau蛋白(Hoffmann et al., 1997)免疫动物而制成。Tau是微管相关蛋白异构物，促进和稳定微管装配，尤其是在轴突中。其中有六个有不同的氨基末端的异构体插入，有不同数量的羧基末端串联重复序列已被识别，tau蛋白可被ERK、GSK-3和CDK5在大约25个位点处被过度磷酸化（1-2）。Tau蛋白磷酸化可降低其与微观的结合能力。神经纤维缠结是阿尔茨海默氏症的一个主要标志，这些缠结是由过度磷酸化tau蛋白组成的成对螺旋细丝束。GSK-3或CDK5对Tau蛋白Ser396的磷酸化可以导致微管的破坏，特别是在阿尔茨海默氏症中。此外，Tau蛋白包涵体还在其它神经退行性疾病中被发现，这些疾病被统称为Tau蛋白病。

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Phospho-Tau (Ser396) (PHF13) Mouse mAb detects endogenous levels of Tau only when phosphorylated at serine 396.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human purified Tau (Hoffmann et al., 1997).

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.