Phospho-(Ser) Kinase Substrate Antibody Sampler Kit

产品编号：CST-9615S 品牌：Cell Signaling Technology 原厂货号：9615S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C

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其它组分：

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb 【子货号：#2325，包装：40 µl，，运保温度：–20°C】

Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody 【子货号：#2981，包装：40 µl，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：–20°C】

Anti-mouse IgG, HRP-linked Antibody 【子货号：#7076，包装：100 µl，，运保温度：–20°C】

Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix 【子货号：#9477，包装：40 µl，，运保温度：–20°C】

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb 【子货号：#9606，包装：40 µl，，运保温度：–20°C】

描述：

各抗体识别内源性特异激酶底物中含磷酸化丝氨酸的蛋白。更多信息请参考此产品的数据页。多克隆抗体使用包含激酶底物基序的磷酸化肽段免疫动物制备，通过蛋白A和肽段亲和色谱纯化。单克隆抗体使用合成的磷酸化MAPK/CDK底物肽段免疫动物制备。鼠单抗（IgM）使用包含磷酸化丝氨酸的14-3-3结合基序的肽段免疫动物制备。Phospho-(Ser) Kinase Substrate Antibody Sampler Kit提供了一种快速经济评估磷酸化丝氨酸激酶底物的方法。该试剂盒包含足够四次Western blot的一抗和二抗。磷酸化丝氨酸激酶和磷酸酶在生物过程中扮演重要角色。每个磷酸化丝氨酸激酶磷酸化特异性基序中的丝氨酸。MAPK和CDK丝氨酸蛋白激酶家族磷酸化脯氨酸残基之前的丝氨酸（1-3）。CDK底物的共同氨基酸序列为(K/R)(S*)PX(K/R)，X代表任意20种氨基酸之一，S*是磷酸化位点（4-6）。MAPK磷酸化含有一致序列PX(S*)P的底物。14-3-3蛋白是参与调解细胞生存、凋亡、增殖和检查点控制的一组高度保守的蛋白家族（7-11）。14-3-3的结合由包含磷酸化丝氨酸的蛋白质介导（12）。两种包含磷酸化丝氨酸的基序在简并磷酸化导向肽库技术中发现，分别RSXS*XP和RXY/FXS*XP（12）。基序1（-3和-2位上精氨酸/赖氨酸和丝氨酸，0位磷酸化丝氨酸，+2位脯氨酸）在很多重要的调节蛋白包括Bad、cdc25C、Fox03A、PKC和cRaf中发现（11,13）。基序2（RXY/FXS*XP）在很多重要的调节蛋白包括cdc25A、cdc25B、PKCγ、IRS-1和BCR中发现（12）。尽管Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody可以结合14-3-3结合基序2，并且不需要+2位上的脯氨酸，它仍然是发现和鉴定潜在包含14-3-3结合基序2蛋白或其它含RXY/FXS*基序蛋白的有力工具。某些激酶底物有特异性的抗体对于研究激酶活性和发现潜在底物具有很高价值。CST开发了能够识别包含MAPK/CDK、CDK或14-3-3可以磷酸化的基序中磷酸化丝氨酸和苏氨酸的这些抗体。通过DELFIA或ELISA证实，此试剂盒中每个磷酸化丝氨酸激酶底物抗体都有特异性的目标激酶底物基序。

原厂资料：

Specificity / Sensitivity

Each antibody detects endogenous levels of phospho-(Ser) proteins of specific kinase substrate groups. For more detailed information please see datasheet for this product.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with phosphopeptides containing the kinase substrate motif and purified by protein A and peptide affinity chromatography.Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides.Mouse monoclonal antibody is produced by immunizing animals with phospho-(Ser) 14-3-3 binding motif peptides.

Description

The Phospho-(Ser) Kinase Substrate Antibody Sampler Kit provides a fast and economical means of evaluating several phospho-Ser kinase substrates. The kit contains enough primary and secondary antibody to perform four Western blot experiments.

Background

Phospho-(Ser) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser) kinase phosphorylates serine within a specific motif. The MAPK and CDK families of serine protein kinases phosphorylate serine followed by proline residue (1-3). The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). MAPK phosphorylates substrates with the concensus sequence PX(S*)P. The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (7-11). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (12). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (12). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FoxO3A, PKC and c-Raf (11, 13). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCγ, IRS-1 and BCR (12). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine within the context of a protein motif that is phosphorylated by MAPK/CDK, CDKs or 14-3-3. As shown by DELFIA or ELISA, each phospho-(Ser) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif.

磷酸化丝氨酸激酶和磷酸酶在生物过程中扮演重要角色。每个磷酸化丝氨酸激酶磷酸化特异性基序中的丝氨酸。MAPK和CDK丝氨

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies.