Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb

• 产品编号：CST-9614L      品牌：CST       原厂货号：9614L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*) (110B7) Rabbit mAb兔单抗识别包含-3位上是精氨酸的磷酸化丝氨酸/苏氨酸的肽段和蛋白。此抗体与包含-5、-3位上是精氨酸的磷酸化丝氨酸/苏氨酸肽段有偏好的交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该单克隆抗体用合成的磷酸化Akt底物肽段免疫动物制备。精氨酸导向的激酶或AGC家族激酶是很重要的激酶，包括cAMP依赖的蛋白激酶（PKA）、cGMP依赖的蛋白激酶（PKG）、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性，-3位上有精氨酸的丝氨酸和苏氨酸（1,2）。Akt在介导细胞内关键反应中扮演重要角色，包括细胞生长和生存、血管生成和转录调节（3-5）。目前已经发现一些Akt底物（如GSK-3、Bad、caspase-9），但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸（6）。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发，用于研究Akt磷酸化调节和精氨酸导向的激酶，也可以用于高通量药物发现。

1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*)(110B7) RmAb recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides .

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.