Phospho-MSK1 (Thr581) Antibody

• 产品编号：CST-9595S      品牌：CST       原厂货号：9595S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MSK1 (Thr581) Antibody兔多抗能检测内源性Thr581位点磷酸化的MSK1蛋白水平。该抗体不能与其他位点磷酸化的MSK1蛋白发生交叉反应，也不能检测磷酸化的RSK1, RSK2和RSK3蛋白。该多克隆抗体通过使用与人源MSK1蛋白苏氨酸581周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。MSK1是一种有丝分裂原和应激活化的蛋白激酶，能够被Erk、p38 MAPK活化，分别应答生长因子和细胞应激反应的刺激（1）。MSK1与RSK类似，它有两个激酶区域，这两个区域由调节链接区域连接（2）。RSK1蛋白中Ser364和Ser381位点的磷酸化对RSK1的活性非常重要。MSK1的Ser360和Ser376位点与RSK1中的这两个位点同源，可能对MSK1的活性也同样重要。

1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
3. Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496-1505.
4. Markou, T. and Lazou, A. (2002) Biochem J 365, 757-63.

原厂资料：

Specificity / Sensitivity

Phospho-MSK1 (Thr581) Antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581. This antibody does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
3. Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496-1505.
4. Markou, T. and Lazou, A. (2002) Biochem J 365, 757-63.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody