SMG-1 (V72) Antibody

• 产品编号：CST-9592S      品牌：CST       原厂货号：9592S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SMG-1 (V72) Antibody 能够检测内源性SMG-1总蛋白水平。该抗体能够和未知起始物的100 kDa 的带交叉反应。通过共聚焦免疫荧光检测，该抗体标记了U-2 OS细胞的线粒体(7)。该多克隆抗体是由合成的针对人SMG-1近氨基末端残基的肽段免疫动物生产的。 SMG-1是磷酸肌醇3激酶相关激酶(PIKK)家族的一员，该家族包括ATM，ATR，mTOR，DNA-PKcs和TRRAP (1,2)。经由DNA损伤激活，SMG-1已经被发现能够磷酸化p53 和hUpf1 (SMG-2) (1-4)。hUpf1是监视复合体的一个亚基，该复合体允许信使RNA物种(包含提前终止密码子PTC)的降解。该过程亦被称作无意义介导的mRNA降解(NMD)，可以阻止蛋白截断体形式的翻译，也能够引起功能获得或者优势阴性物种。NMD在正常细胞环境中发生，同时也应答DNA损伤(5,6)。SMG-1亦被认为能够影响细胞死亡受体信号和保护细胞外部诱导凋亡性细胞死亡(7)。

1. Denning, G. et al. (2001) J Biol Chem 276, 22709-14.
2. Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
3. Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98.
4. Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200.
5. Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705.
6. Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8.
7. Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84.

原厂资料：

Specificity / Sensitivity

SMG-1 (V72) Antibody detects endogenous levels of total SMG-1 protein. The antibody cross-reacts with a 100 kDa band of unknown origin. By confocal immunofluorescence, the antibody labels the mitochondria of U-2 OS cells (7). 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1.

Background

SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7). 

1. Denning, G. et al. (2001) J Biol Chem 276, 22709-14.
2. Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
3. Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98.
4. Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200.
5. Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705.
6. Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8.
7. Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.