Mouse Interleukin-17B (mIL-17B)

• 产品编号：CST-9584LF      品牌：CST       原厂货号：9584LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

重组小鼠IL-17B (mIL-17B) His21-Phe180 (Accession #NP_062381)蛋白在大肠杆菌中表达。重组mIL-17B蛋白在氨基末端有Met残基，其分子量据推算为18,186Da。DTT-还原和未还原的蛋白接近19 kDa 多肽迁移的位置。重组 mIL-17B蛋白的氨基末端MHPRN的序列通过氨基酸测序得到。通过对6 μg 还原 (+) 和 非还原 (-) 的重组 mIL-17B进行SDS-PAGE检测，纯度大于98%。所有批次的纯度均大于98%。mIL-17B 的活性是通过在 ELISA实验中对 IL-17RB 的结合能力来确定的，每个批次的半数最大结合浓度为 0.1-1.0 µg/ml。每微克mIL-17B的内毒素含量小于 0.01 ng。

有载体: 每微克mIL-17B溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜后冻干。

无载体：每微克mIL-17B溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜后冻干。

IL-17B 在结构上是 IL-17 家族中的一员(1)。与IL-17 家族的其它成员不同, IL-17B 不是共价结合的同型二聚体(1,2)。 IL-17B 在软骨细胞和神经细胞中表达，并结合到IL-17RB受体上 (1-4)。 人源的IL-17B 能诱导单核细胞 THP-1的 TNF-α 和 IL-1β的表达，并参与体内噬中性粒细胞的招募(3)。然而，IL-17B的确切的生物学功能仍然不清楚。

原厂资料：

Source / Purification

Recombinant mouse IL-17B (mIL-17B) His21-Phe180 (Accession #NP_062381) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-17B has a Met on the amino terminus and has a calculated MW of 18,186. DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides. The expected amino-terminal MHPRN of recombinant mIL-17B was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-17B. All lots are greater than 98% pure. 

Bioactivity

The activity of mIL-17B was assessed by its ability to bind to IL-17RB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 0.1-1.0 µg/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-17B. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-17B is a member of the IL-17 family of structurally related proteins (1). Unlike other IL-17 family members, IL-17B is a non-covalently linked homodimer (1,2). IL-17B is expressed by chondrocytes and neurons, and binds to the IL-17RB receptor (1-4). Human IL-17B has been shown to induce TNF-α and IL-1β from monocytic THP-1 cells and may be involved in neutrophil recruitment in vivo (3). However, the exact biological functions of IL-17B remain elusive.

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.