Phospho-eNOS (Thr495) Antibody

• 产品编号：CST-9574S      品牌：CST       原厂货号：9574S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-eNOS (Thr495) AntibodyAntibody可以检测Thr495位磷酸化的内源性总eNOS蛋白。多克隆抗体是采用合成的与人eNOS的Thr495周围序列相对应的磷酸肽免疫动物而生产的。抗体用蛋白A和肽亲和层析来纯化的。内皮一氧化氮合酶（eNOS）是心血管系统的一种重要的酶。它可以催化合成一氧化氮（NO）。NO是一种血压，血管重建和生成的重要的调节因子（1,2）。eNOS的活性受多个位点磷酸化的调控。研究最为透彻的两个位点是激活位点Ser1177 和抑制位点Thr495（3）。几种蛋白激酶包括Akt/PKB, PKA 和AMPK都可以在不同的刺激下通过 Ser1177位点的磷酸化来激活eNOS的活性（4,5）。相反，血管舒缓激肽和过氧化氢通过促进Ser1177的磷酸化和Thr495的去磷酸化来激活eNOS的活性（6,7）。

1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
4. Dimmeler, S. et al. (1999) Nature 399, 601-605.
5. Fulton, D. et al. (1999) Nature 399, 597-601.
6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-eNOS (Thr495) Antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
4. Dimmeler, S. et al. (1999) Nature 399, 601-605.
5. Fulton, D. et al. (1999) Nature 399, 597-601.
6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody