Phospho-β-Catenin (Ser45) Antibody

• 产品编号：CST-9564S      品牌：CST       原厂货号：9564S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化 β- catenin (Ser45)抗体只识别Ser45磷酸化的内源性β- catenin。此抗体不识别其他位点磷酸化的β- catenin。多克隆抗体由合成的磷酸化肽段免疫动物产生，合成的磷酸化肽段与人源β- catenin Ser45氨基酸残基序列一致。抗体由蛋白质A和肽段亲和层析纯化得到。β-catenin是Wnt信号通路下游的重要效应分子（1）。在脊椎动物体内它涉及到两个重要的生物过程：早期胚胎发育（2）和肿瘤发生（3）。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化（4-6）。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41，并促使其进一步被降解（7）。这些位点发生突变会提高β-catenin蛋白稳定性，并且已在多种癌细胞株中发现这些突变（8）。

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Dog, Pig

Specificity / Sensitivity

Phospho-β-Catenin (Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at serine 45. It does not recognize β-catenin phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody