Phospho-Smad1 (Ser206) Antibody

• 产品编号：CST-9553S      品牌：CST       原厂货号：9553S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Smad1 (Ser206)抗体仅能识别206位丝氨酸磷酸化的内源性Smad1蛋白。此抗体与家族内其他蛋白没有交叉反应。合成对应Smad1 蛋白的邻近206位苏氨酸的氨基酸残基序列一致的磷酸化肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。骨形成蛋白由一大类信号分子组成，这些分子调控包括形态发生、细胞命运定向、增殖、分化以及凋亡在内的广泛重要过程[1,2]。BMP 受体为TGF-β家族丝氨酸/苏氨酸受体成员。配基的结合诱导多聚化、自磷酸化和相应受体的激活[3-5]。它们随后磷酸化Smad1蛋白的C-末端SSXS模体中的463位丝氨酸和465位丝氨酸，且磷酸化Smad5和Smad8中相应的位置。这些磷酸化的Smad与共激活的Smad4形成二聚体，转移到核内激活靶基因的转录[5]。促分裂原活化蛋白激酶MAPK和周期蛋白依赖性蛋白激酶8和9在Smad1的连接区域磷酸化相应残基包括丝氨酸206。进而募集Smurf1到接头区域导致Smad1的降解[6]。这一位点的磷酸化也招募YAP到接头区域促进Smad1的转录激活[7]。MAPKs磷酸化Smad1蛋白包括206位丝氨酸的链接区域，通过滞留Smad1在细胞质并降解的方式抑制Smad1功能。

1. Hogan, B.L. (1996) Genes Dev 10, 1580-94.
2. Hoodless, P.A. et al. (1996) Cell 85, 489-500.
3. Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92.
4. Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Sapkota, G. et al. (2007) Mol Cell 25, 441-54.
7. Alarcón, C. et al. (2009) Cell 139, 757-69.
8. Sapkota, G. et al. (2007) Mol. Cell 25, 441-454.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-Smad1 (Ser206) Antibody detects endogenous levels of Smad1 only when phosphorylated at Ser206. No cross reactivity was detected with other famly members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser206 of Smad1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).

MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).MAPKs phosphorylate the linker region of Smad1, including Ser206, and inhibit Smad1 activity through cytoplasmic retention and degradation (6).

1. Hogan, B.L. (1996) Genes Dev 10, 1580-94.
2. Hoodless, P.A. et al. (1996) Cell 85, 489-500.
3. Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92.
4. Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Sapkota, G. et al. (2007) Mol Cell 25, 441-54.
7. Alarcón, C. et al. (2009) Cell 139, 757-69.
8. Sapkota, G. et al. (2007) Mol. Cell 25, 441-454.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.