Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb

• 产品编号：CST-9549L      品牌：CST       原厂货号：9549L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb兔单抗可以检测在Ser380, Thr382和Thr383位点发生磷酸化的内源性PTEN蛋白。单克隆抗体是采用人类PTEN蛋白Ser380, Thr382 和Thr383周围相对应残基的合成磷酸肽段免疫动物生产的。PTEN（phosphatase and tensin homologue deleted on chromosome ten），也称为MMAC（ (mutated in multiple advanced cancers)磷酸酶，是一种存在于多种人类癌症中的肿瘤抑制因子（1）。PTEN编码一个403个氨基酸组成的多肽，最初被认为是一个双特异性蛋白磷酸酶（2）。PTEN的主要底物是3磷酸肌醇激酶（PI3K）激活转化生成的肌醇磷脂（3）。PTEN是PI3K/Akt信号通路的一个主要的负调因子（1,4,5）。PTEN有一个羧基端的非催化调控的结构域，其中含有3个可以调节PTEN的稳定性，也可能影响其生物活性的磷酸化位点（Ser380，Thr382，Thr383）（6,7）。PTEN调节p53蛋白水平和活性（8），并在趋化过程中参与G蛋白偶联信号转导（9,10）。

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN only when phosphorylated at Ser380, Thr382 and Thr383.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.