PathScan? Total Oct-4A Sandwich ELISA Kit

• 产品编号：CST-9539C      品牌：CST       原厂货号：9539C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

PathScan^® Total Oct-4A Sandwich ELISA Kit能够检测内源性水平的总Oct-4A蛋白。本试剂盒用来检测指定物种的蛋白，即通过内部测试确定的指定物种蛋白，但也可能检测来自其他物种的同源蛋白。PathScan® Total Oct-4A Sandwich ELISA Kit是固相夹心酶联免疫吸附试验（ELISA），能够检测内源性水平的Oct-4A。用Oct-4A兔抗包被微孔板。细胞裂解液孵育后，Oct-4A蛋白由包被的抗体捕获。充分洗涤后，加入Oct-4A鼠检测抗体检测捕获的Oct-4A蛋白。随后用抗鼠IgG, HRP-标记的抗体识别结合的检测蛋白。加入HRP底物TMB进行显色。显色的吸光度值与Oct-4A蛋白的量成正比。试剂盒中的抗体是针对试剂盒定制的。Oct-4 (POU5F1)是在未分化的胚胎干细胞、胚胎生殖细胞中高表达的转录因子（1）。包括Oct-4, Nanog和 Sox2在内的关键因子网络对于维持多能性的潜能是必须的，已经证明，Oct-4的下调会触发细胞分化(2,3)。研究表明，Oct-4是一个有用的生殖细胞肿瘤标记物（4）。Oct-4存在两个剪接变异体，即Oct-4A 和Oct-4B (5). 最近的研究表明，Oct-4A亚型有赋予和维持多能性的能力，而Oct-4B可能存在在一些体细胞而非多能干细胞内（6,7）。

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

原厂资料：

Specificity / Sensitivity

PathScan^® Total Oct-4A Sandwich ELISA Kit detects endogenous levels of total Oct-4A protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total Oct-4A Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Oct-4A. An Oct-4A Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, Oct-4A protein is captured by the coated antibody. Following extensive washing, an Oct-4A Mouse Detection Antibody is added to detect the captured Oct-4A protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of Oct-4A. 
 Antibodies in kit are custom formulations specific to kit.

Background

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).