Phospho-cdc25C (Ser198) Antibody

• 产品编号：CST-9529S      品牌：CST       原厂货号：9529S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-cdc25C (Ser198) Antibody能够识别外源性转染的丝氨酸（198位）磷酸化的cdc25C蛋白。该多克隆抗体是由合成的人源的针对cdc25C蛋白丝氨酸(198位)磷酸化肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。 cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。在细胞分裂前期，polo-样激酶1(PLK1)磷酸化cdc25C蛋白丝氨酸198位，可使cdc25C从胞浆转位至胞核，磷酸化cdc25C在胞核内与cdc2/cyclin B相互作用，使有丝分裂的其他时相有序进行。

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
6. Toyoshima-Morimoto, F. et al. (2002) EMBO Rep. 3, 341-348.

原厂资料：

Specificity / Sensitivity

Phospho-cdc25C (Ser198) Antibody detects levels of cdc25C only when phosphorylated at Ser198 and only when derived from a transfected DNA construct.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser198 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). During prophase, polo-like kinase 1 (PLK1) phosphorylates cdc25C at Ser198, causing translocation from the cytoplasm to the nucleus, where cdc25C can interact with cdc2/cyclin B to allow for progression through the remaining stages of mitosis (6).

1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
6. Toyoshima-Morimoto, F. et al. (2002) EMBO Rep. 3, 341-348.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.