Cleaved Caspase-9 (Asp315) Antibody (Human Specific)

• 产品编号：CST-9505P      品牌：Cell Signaling Technology       原厂货号：9505P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源性的人源caspase-9(Asp315)裂解大片段(37 kDa with prodomain/17 kDa)的，与全长 Caspase-9以及其他caspase裂解片段无交叉反应.该单克隆抗体是采用合成的人caspas-9 Asp315 周围残基相对应的多肽免疫的动物而制备。抗体通过蛋白A和肽段亲和层析而纯化.Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后，细胞色素C从线粒体释放，这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化，包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处，产生p37亚单位，可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用，包括 caspase-3 and caspase-7,并启动凋亡级联反应，最终导致凋亡。

1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
3. Liu, X. et al. (1996) Cell 86, 147-157.
4. Li, P. et al. (1997) Cell 91, 479-489.
5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-9 (Asp315) Antibody (Human Specific) detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
3. Liu, X. et al. (1996) Cell 86, 147-157.
4. Li, P. et al. (1997) Cell 91, 479-489.
5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.