Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb

• 产品编号：CST-9496S      品牌：CST       原厂货号：9496S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb 兔单抗能够检测人caspase-8 Asp391（亚型A，亚型B是在Asp374位）剪切的片段，该抗体可以检测包含带有P18亚单位的前体结构域和独立的P18 亚单位。该单克隆抗体是采用合成的人caspase-8蛋白Asp391周围残基相对应的多肽免疫动物生产制备。CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8，并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases，如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化，包括DNA的片段化和细胞固缩。

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human Caspase-8.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibod