PKC412

• 产品编号：CST-9493S      品牌：CST       原厂货号：9493S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

提供的PKC412为冻干粉。对于1mM浓度贮存液，在1mg冻干粉中加入1.75 mL DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是0.1-10 μM，处理18-48小时。能溶于DMSO（100 mg/ml）和加热的乙醇（5 mg/mL）。难溶于水，最大溶解度约为10-20 μM。星状孢子素类似物，PKC412，能抑制许多种丝氨酸/苏氨酸和酪氨酸激酶的活性，包括PKC(IC50 = 50 nM)，环AMP依赖性蛋白激酶(IC50 = 2.4 µM)，S6激酶(IC50 = 5.0 µM)和EGFR（表皮生长因子受体）酪氨酸激酶(IC50 = 3.0 µM)（1）。PKC412对FLT3也有很强的抑制作用，FLT3能引起G1细胞周期阻滞和细胞凋亡（2）。

原厂资料：

Molecular Characterization

C35H30N4O4

Purity

>99%

Directions for Use

PKC412 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute 1 mg in 1.75 mL DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.1-10 μM for 18-48 hours. Soluble in DMSO at 100 mg/mL; soluble in ethanol at 5 mg/mL with warming; very poorly soluble in water with maximum solubility estimated to be about 10-20 μM.

Background

The staurosporine analog, PKC412, inhibits a variety of serine/threonine and tyrosine kinases including PKC (IC50 = 50 nM), cyclic AMP-dependent protein kinase (IC50 = 2.4 µM), S6 kinase (IC50 = 5.0 µM), and EGFR (epidermal growth factor receptor) tyrosine kinase activity (IC50 = 3.0 µM) (1). PKC412 has also demonstrated stong inhibitory effects on FLT3, which causes G1 cell cycle arrest and apoptosis (2).

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles