Caspase-7 Antibody

• 产品编号：CST-9492S      品牌：CST       原厂货号：9492S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspase-7 Antibody 能够检测内源性的caspase-7 蛋白全长（35KD）和198位天冬氨酸出裂解的大片段。该多克隆抗体是采用合成的人caspase-7 裂解位点周围氨基序列相对应的多肽免疫动物而生产制备，通过蛋白A和亲和色谱法纯化。Caspase-7 (CMH-1, Mch3, ICE-LAP3)被鉴定为凋亡的主要执行者（2-4）。Caspase-7是一种效应酶（与caspase-2和caspase-3），意味着它在胞内裂解发挥必要机制，而不是激活其它caspases（5-8）。Caspase-7通过一系列酶裂解发挥作用，包括caspases-3、-6、-8、-9 和 颗粒酶B(1,4,5)。Caspase-7一旦活化，它能裂解许多与caspase-3相同的底物，包括核糖体聚合酶，或者PARP（2,4）。

1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

原厂资料：

Specificity / Sensitivity

Caspase-7 Antibody detects endogenous levels of both full length caspase-7 (35 kDa) and the large fragment of cleaved caspase-7 following cleavage at aspartic acid 198 (20 kDa). The antibody does not recognize other caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4).

1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody