Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab? Rabbit mAb mix

• 产品编号：CST-9477S      品牌：CST       原厂货号：9477S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb兔多抗识别KS*P基序中磷酸化的丝氨酸。此抗体与包含磷酸化苏氨酸和磷酸化丝氨酸的肽段或蛋白没有交叉反应。该单克隆抗体用合成的磷酸化CDK的底物库肽段免疫动物制备。该抗体是两个兔单克隆的混合，以便可以覆盖更大的反应范围。Cyclin-dependent kinases (CDKs)是一个丝氨酸苏氨酸激酶家族，以严格而有序的方式通过结合并磷酸化目标蛋白调控细胞周期进程（1）。CDK的底物是脯氨酸导向的。CDK底物的共有氨基酸序列为(K/R)(S*)PX(K/R)，X代表任意20种氨基酸之一（2-4），S*是磷酸化位点。Phospho-(Ser) CDKs Substrate Antibody识别共有基序的磷酸化CDK底物，为CDK靶标的发现和鉴定提，及高通量（HTS）药物筛查潜在激酶调节者提供了强大的工具。

1. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
2. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
3. Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb recognizes phospho-serine in a KS*P motif. The antibody does not cross-react with phospho-threonine or phospho-tyrosine containing peptides/proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phospho-CDK substrate peptide library. The antibody is formulated from two rabbit monoclones in order to cover a broad range of reactivity.

Background

Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.

1. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
2. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
3. Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody