FoxO1 (L27) Antibody

• 产品编号：CST-9454S      品牌：CST       原厂货号：9454S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxO1 (L27)Antibody可以检测外源性和内源性总Fox01蛋白。此抗体内源性Fox04或Fox03a发生交叉反应， 此抗体可以与大小95kDa的未知蛋白发生交叉反应。多克隆抗体是采用来源于鼠Fox01蛋白氨基末端的氨基酸相对应的合成肽段免疫动物生产的。抗体由protein A和肽亲和层析法纯化。Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生（1-3）。在这个家族中，三个成员（FoxO1，FoxO4和FoxO3a）与线虫的同源基因DAF-16具有序列相似度，而DAF-16介导包括IGFR1，PI3K和Akt在内的信号通路（4-6）。活化的forkhead成员作为肿瘤抑制因子，促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调，而这个上调过程可以被PI3K负调控（7）。forkhead转录因子被Akt在Thr24，Ser256以及Ser319磷酸化后而失活，导致出核转运与转录因子活性的抑制（8），最终导致增殖增加。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SirT1)抑制（9）。

1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
7. Seoane, J. et al. (2004) Cell 117, 211-23.
8. Arden, K.C. (2004) Mol Cell 14, 416-8.
9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

FoxO1 (L27) Antibody detects endogenous levels of total FoxO1 protein and does not cross-react with endogenous FoxO3a or FoxO4. The antibody also cross-reacts with an unidentified protein at 95 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids within the amino terminus of mouse FoxO1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
7. Seoane, J. et al. (2004) Cell 117, 211-23.
8. Arden, K.C. (2004) Mol Cell 14, 416-8.
9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody