SMARCA1 Antibody

• 产品编号：CST-9450S      品牌：CST       原厂货号：9450S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SMARCA1 Antibody能够检测内源性SMARCA1总蛋白水平。通过合成的与人源SMARCA1蛋白氨基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。SMARCA1 (SNF2L)是ISWI (imitation switch) ATPases的两个直系同源物之一，其由哺乳动物基因编码(1)。 ISWI染色质重塑复合物首先被鉴定在果蝇中，并且在体外被显示去改造和改变核小体间隔(2)。SMARCA1是NURF(nucleosome remodeling factor)和CERF(CECR2-containing remodeling factor) 复合物的催化亚单位(3-5)。NURF复合物在神经生理学中起到重要作用，通过促进神经突起生长和调节Engrailed同源异型基因，该基因涉及到在中后脑中的神经元的发育(3)。NURF也是被认为是涉及来自胸腺的T细胞成熟，这通过调节染色质结构和T细胞发育的基因表达 (6)。NURF复合物的最大亚基BPTF对于中胚层、内胚层和外胚层组织谱系是需要的，这认为在小鼠胚胎中SMARCA1的功能是促进胚层的发育 (7)。CECR2是一个SMARCA1相互作用的蛋白质，由CECR2的缺失引起的CERF复合物的分解与露脑性神经索缺陷有关联，这联系着与神经胚形成的调节有关的CERF复合物(4)。

1. Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem 77, 1145-56.
2. Erdel, F. and Rippe, K. (2011) FEBS J 278, 3608-18.
3. Barak, O. et al. (2003) EMBO J 22, 6089-100.
4. Banting, G.S. et al. (2005) Hum Mol Genet 14, 513-24.
5. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
6. Landry, J.W. et al. (2011) Genes Dev 25, 275-86.
7. Landry, J. et al. (2008) PLoS Genet 4, e1000241.

原厂资料：

Specificity / Sensitivity

SMARCA1 Antibody recognizes endogenous levels of total SMARCA1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMARCA1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SMARCA1 (SNF2L) is one of the two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). The ISWI chromatin remodeling complexes were first identified in Drosophila and have been shown to remodel and alter nucleosome spacing in vitro (2). SMARCA1 is the catalytic subunit of the NURF (nucleosome remodeling factor) and CERF (CECR2-containing remodeling factor) complexes (3-5). The NURF complex plays an important role in neuronal physiology by promoting neurite outgrowth and regulation of Engrailed homeotic genes that are involved in neuronal development in the mid-hindbrain (3). NURF is also thought to be involved in the maturation of T cells from thymocytes by regulating chromatin structure and expression of genes important for T cell development (6). The largest subunit of the NURF complex, BPTF, is required for proper development of mesoderm, endoderm, and ectoderm tissue lineages, suggesting a role for SMARCA1 in the development of the germ layers in mouse embryo (7). Disruption of the CERF complex by deletion of CECR2, an interacting partner of SMARCA1, is associated with the neural tube defect exencephaly, linking the CERF complex with regulation of neurulation (4).

1. Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem 77, 1145-56.
2. Erdel, F. and Rippe, K. (2011) FEBS J 278, 3608-18.
3. Barak, O. et al. (2003) EMBO J 22, 6089-100.
4. Banting, G.S. et al. (2005) Hum Mol Genet 14, 513-24.
5. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
6. Landry, J.W. et al. (2011) Genes Dev 25, 275-86.
7. Landry, J. et al. (2008) PLoS Genet 4, e1000241.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.