BCAT2 Antibody

• 产品编号：CST-9432S      品牌：CST       原厂货号：9432S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BCAT2 Antibody能够检测内源性BCAT2总蛋白水平。该多克隆抗体是由合成的人源BCAT2蛋白N末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。BCAT1和BCAT2分别是胞浆和线粒体支链氨基转移酶（1,2）。研究显示晚期大肠癌患者的BCAT1蛋白发生远处转移（3）。小鼠BCAT2蛋白的缺失会升高血浆支链氨基酸水平，减少肥胖和体重，增加能量消耗，这表明了其对于调节胰岛素敏感性的作用（4）。

1. Bledsoe, R.K. et al. (1997) Biochim Biophys Acta 1339, 9-13.
2. Suryawan, A. et al. (1998) Am J Clin Nutr 68, 72-81.
3. Yoshikawa, R. et al. (2006) World J Gastroenterol 12, 5884-9.
4. She, P. et al. (2007) Cell Metab 6, 181-94.

原厂资料：

Specificity / Sensitivity

BCAT2 Antibody recognizes endogenous levels of total BCAT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BCAT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

BCAT1 and BCAT2 are cytosolic and mitochondrial branched chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids, reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4).

1. Bledsoe, R.K. et al. (1997) Biochim Biophys Acta 1339, 9-13.
2. Suryawan, A. et al. (1998) Am J Clin Nutr 68, 72-81.
3. Yoshikawa, R. et al. (2006) World J Gastroenterol 12, 5884-9.
4. She, P. et al. (2007) Cell Metab 6, 181-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.