Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific)

• 产品编号：CST-9429S      品牌：CST       原厂货号：9429S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) 抗体能够检测内源性水平的小鼠和大鼠 Asp387剪切的caspase-8。该抗体能够检测剪切产物包括含有P18(p43)亚单位的前体结构域，也可以检测独立的P18亚单位。该多克隆抗体是采用合成的针对啮齿类动物caspase-8蛋白Asp387剪切位点周围残基相对应的多肽免疫动物而生产制备。CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8，并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases，如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化，包括DNA的片段化和细胞固缩。

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.