Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose^? Bead Conjugate)

• 产品编号：CST-9419S      品牌：CST       原厂货号：9419S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)在磷酸化酪氨酸蛋白免疫共沉淀分析中有用。P-Tyr-100通过碳水化合物共轭交联到琼脂糖酰肼珠而固定。此抗体与其它磷酸化丝氨酸或苏氨酸的蛋白没有交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该单克隆抗体用包含磷酸化酪氨酸的肽段免疫动物制备。不要在PhosphoScan^®方法中使用IAP。IAP固定抗体为PhosphoScan^® Kit专用。酪氨酸磷酸化在细胞信号转导中扮演重要角色（1）。在肿瘤中，失调的酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成（2）。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值（3,4）。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体在高通量药物发现中为研究酪氨酸磷酸化和监测酪氨酸激酶活性提供了卓越的高灵敏的新工具。

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

原厂资料：

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate) is useful for immunoprecipitation of tyrosine-phosphorylated proteins. P-Tyr-100 is immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. The antibody does not cross-react with proteins phosphorylated on serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides.

Description

Do not use for IAP in the PhosphoScan^® method. IAP immobilized antibody is specific to the PhosphoScan^® Kit.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.