SignalSilence? Stat6 siRNA II

• 产品编号：CST-9404S      品牌：CST       原厂货号：9404S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® Stat6 siRNA II能够抑制人、小鼠、大鼠和猴的Stat6的表达。来自Cell Signaling Technology (CST)的SignalSilence^® Stat6 siRNA II可以帮助研究者通过RNA干扰特异性地抑制Stat6的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Stat6 siRNA II 转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中的siRNA的终浓度为100nM。受到JAK激活后, Stat6进入核中并调控细胞因子诱导的基因表达。Stat6通过在Tyr641位点的磷酸化被激活，这对IL-4 和 IL-13的应答是必需的(1-4)。另外，在B细胞中Stat6被 IFN-α激活并与Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶2A(PP2A)也参与到 IL-4介导的 Stat6信号通路调控中 (7)。

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

SignalSilence® Stat6 siRNA II inhibits human, mouse, rat, and monkey Stat6 expression.

Description

SignalSilence® Stat6 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Stat6 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

注意事项：

Storage: Stat6 siRNA II is supplied in RNAse-free water. Aliquot
and store at -20ºC.