SignalSilence? CK2α siRNA I (Rodent Specific)

• 产品编号：CST-9400S      品牌：CST       原厂货号：9400S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® CK2α siRNA I (Rodent Specific)可以抑制小鼠和大鼠CK2α表达。.Cell Signaling Technology (CST) 公司的SignalSilence® CK2α siRNA I (Rodent特异) 帮助研究人员使用RNA干扰技术特异性抑制ERCC1表达，该技术通过向细胞内导入双链RNA分子以选择性的抑制某些基因的表达。所有CST公司的SignalSilence® siRNA都经过了严格的内部测试，western分析显示能够能够降低目标蛋白的表达。寡核苷酸合成经过了三苯甲基的逐碱基分析以确保耦合效率。随后经过亲和固相萃取纯化。退火的RNA双链在经过质谱分析以确保双链的准确性。新批次都会经过质谱分析与之前的批次进行比对以保证不同批次之间的稳定性。CST建议转染100 nM SignalSilence® CK2α siRNA I (Rodent特异)，48-72小时后裂解细胞。转染的步骤请参阅转染试剂的说明书。关于使用问题，可以随时咨询CST。 每管试剂能进行100次转染，该转染次数以总体积为300μl 的1个24孔板孔中siRNA终浓度100 nM来进行计算。CK2（也称为酪蛋白激酶II)）是一种有超过300个底物，高度保守的蛋白激酶，调控细胞生长，细胞死亡和细胞生存。CK2涉及到UV辐射引发的DNA损伤，靶向XRCC1(1)和BRCA1（2）以及调控p53抑癌蛋白发挥作用（3）。并且，CK2还能激活NF-κB（4）。UV照射会刺激CK2介导的IκBα羧基端多个残基的磷酸化，导致IκBα通过蛋白酶体降解以及NF-κB释放转位到细胞核发挥作用。CK2也在多种癌症疾病（5）以及神经障碍疾病例如阿尔兹海默氏病和帕金森病（6）中异常表达。从结构上看，CK2是一个包含有2个催化亚基（α或α’）和2个调控β亚基的多聚体蛋白复合物（7）。CK2表达广泛且持续激活（7）。在CK2α 和CK2β上发现了细胞周期依赖的丝氨酸-脯氨酸磷酸化位点，Src-相关激酶c-Fgr对CK2α的255位酪氨酸有很强的磷酸化作用（8,9）。

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

SignalSilence® CK2α siRNA I (Rodent Specific) inhibits mouse and rat CK2α expression.

Description

SignalSilence® CK2α siRNA I (Rodent Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CK2α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

ections for Use

CST recommends transfection with 100 nM SignalSilence® CK2α siRNA I (Rodent Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimer's and Parkinson's diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α') and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9).

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

注意事项：

Storage: CK2α siRNA I (Rodent Specific) is supplied in RNAsefree
water. Aliquot and store at -20ºC.