SP1 (D4C3) Rabbit mAb

• 产品编号：CST-9389S      品牌：CST       原厂货号：9389S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SP1 (D4C3) Rabbit mAb兔单抗能够检测内源性SP1总蛋白水平。该单克隆抗体是采用合成的与人源SP1蛋白的Pro593残基周围序列相对应的肽段免疫动物而生产的。Specificity protein 1 (SP1)是一个广泛表达的转录因子，它属于包含DNAC2H2-type锌指的DNA结合蛋白。SP1高亲和力地结合到GC富含区，并且调节哺乳动物基因的表达(1,2)。它与许多其它转录因子例如c-Myc、Egr1和Stat1以及基本的转录组件相互作用。SP1与在染色质修饰中染色质修饰因子例如组蛋白去乙酰酶(HDACs)和p300。SP1的转录活性和稳定性通过翻译后修饰被调节，包括磷酸化、乙酰化、泛素化和糖基化(3)。在胰岛素处理下SP1的糖基化导致细胞核定位的增加，而胰高血糖素处理可增加细胞质的SP1水平(4-6)。研究者发现在阿尔茨海默病(AD)中SP1是有高表达的。

1. Kadonaga, J.T. et al. (1987) Cell 51, 1079-90.
2. Song, J. et al. (2003) Int J Mol Med 11, 547-53.
3. Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8.
4. Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91.
5. Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50.
6. Solomon, S.S. et al. (2008) Life Sci 83, 305-12.
7. Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504.

原厂资料：

Specificity / Sensitivity

SP1 (D4C3) Rabbit mAb recognizes endogenous levels of total SP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro593 of human SP1 protein.

Background

Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, Egr1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitylation, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimer's disease (7).

1. Kadonaga, J.T. et al. (1987) Cell 51, 1079-90.
2. Song, J. et al. (2003) Int J Mol Med 11, 547-53.
3. Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8.
4. Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91.
5. Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50.
6. Solomon, S.S. et al. (2008) Life Sci 83, 305-12.
7. Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody