Phospho-Threonine Antibody (P-Thr-Polyclonal)

• 产品编号：CST-9381S      品牌：CST       原厂货号：9381S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Threonine Antibody (P-Thr-Polyclonal)可以识别苏氨酸残基磷酸化的蛋白质和肽段，与周围氨基酸序列磷酸化情况无关。该抗体是磷酸化特异性的，可能与其它包含磷酸化丝氨酸的序列发生交叉反应。在ELISA中，其可以识别多种苏氨酸磷酸化的肽段，在二维凝胶Western Blot中，其可以识别很多推测的苏氨酸磷酸化的蛋白。CST推荐使用Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391来检测包含脯氨酸跟随苏氨酸的蛋白质。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）.该多克隆抗体用合成的含磷酸化苏氨酸的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。细胞内很多蛋白质的动态行为，包括分子相互作用的调节（1），亚细胞定位（2）和转录调节（3）都由多种转录后修饰调控（4）。对这些转录后修饰特异性的抗体对理解正常和病理的分子和细胞行为非常有价值。大部分蛋白修饰抗体设计出来与修饰的氨基酸残基发生反应（如磷酸化苏氨酸、磷酸化酪氨酸、乙酰化赖氨酸、硝基化酪氨酸），而不考虑这些残疾在序列中的位置。这种不考虑上下文而仅识别修饰后残基的能力给予这些抗体广泛的识别可能，赋予其识别上百种不同蛋白质的能力。这种广泛的识别使这些抗体在复杂分析和目标筛查的过程中具有价值。蛋白激酶是真核细胞调节蛋白中最多的蛋白之一；哺乳动物中有超过500个独立的激酶基因编码这些蛋白（5,6）。尽管它们在真核生物的激酶中很重要，但对它们的生理靶标还相对知之甚少。Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381和Phospho-Threonine (42H4) mAb #9386为发现丝氨酸/苏氨酸激酶、监测和鉴定体外苏氨酸磷酸化反应、高通量丝氨酸/苏氨酸激酶药物发现提供了有力工具。

1. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
2. Appella, E. and Anderson, C.W. (2001) Eur J Biochem 268, 2764-72.
3. Jenuwein, T. and Allis, C.D. (2001) Science 293, 1074-80.
4. Krishna, R.G. and Wold, F. (1993) Adv Enzymol Relat Areas Mol Biol 67, 265-98.
5. Venter, J.C. et al. (2001) Science 291, 1304-51.
6. Manning, G. et al. (2002) Science 298, 1912-34.

原厂资料：

Specificity / Sensitivity

Phospho-Threonine Antibody (P-Thr-Polyclonal) detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides, and by 2D gel Western blot analysis, it recognizes a large number of presumably threonine-phosphorylated proteins. CST recommends the use of Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-Thr-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. 
 General protein modification antibodies are designed to react with modified amino acid residues (e.g., phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.Protein kinases are among the most abundant eukaryotic regulatory proteins; over 500 separate kinase genes are encoded in mammalian genomes (5,6). In spite of the importance of kinases in eukaryotic biology, relatively few of their physiological targets are known. Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 and Phospho-Threonine (42H4) mAb #9386 provide powerful tools for discovering targets of serine/threonine kinases, for monitoring and characterizing in vitro threonine phosphorylation reactions as well as for high throughput Ser/Thr kinase drug discovery.

1. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
2. Appella, E. and Anderson, C.W. (2001) Eur J Biochem 268, 2764-72.
3. Jenuwein, T. and Allis, C.D. (2001) Science 293, 1074-80.
4. Krishna, R.G. and Wold, F. (1993) Adv Enzymol Relat Areas Mol Biol 67, 265-98.
5. Venter, J.C. et al. (2001) Science 291, 1304-51.
6. Manning, G. et al. (2002) Science 298, 1912-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody