Rab7 (D95F2) XP? Rabbit mAb

• 产品编号：CST-9367P      品牌：Cell Signaling Technology       原厂货号：9367P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab7 (D95F2) XP® Rabbit mAb兔单抗识别内源Rab7总蛋白水平。该单克隆抗体用与人类Rab7蛋白中Glu188位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Rab7和Rab9是小Rab GTP酶Ras超家族成员（1）。两个蛋白都定位于晚期内体，但是能够执行不同的功能。Rab7与RIPL效应蛋白结合，控制从早期到晚期内体和到溶酶体的膜转运（2,3）。Rab7也能够帮助调节胞内生长因子受体的内吞转运和降解（3,4），以及吞噬体和吞噬泡的成熟（4-6）。Rab9和其效应蛋白p40和TIP47相互作用（7,8），促进MPR（甘露糖6-磷酸受体）结合的溶酶体酶在晚期内体到高尔基体反面的转运（9,10）。

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
3. Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
4. Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
5. Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
6. Méresse, S. et al. (1999) EMBO J 18, 4394-403.
7. Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
8. Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
9. Lombardi, D. et al. (1993) EMBO J 12, 677-82.
10. Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.

原厂资料：

Specificity / Sensitivity

Rab7 (D95F2) XP® Rabbit mAb detects endogenous levels of total Rab7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein.

Background

Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
3. Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
4. Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
5. Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
6. Méresse, S. et al. (1999) EMBO J 18, 4394-403.
7. Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
8. Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
9. Lombardi, D. et al. (1993) EMBO J 12, 677-82.
10. Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody