Phospho-Stat6 (Tyr641) Antibody

• 产品编号：CST-9361P      品牌：Cell Signaling Technology       原厂货号：9361P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Stat6 (Tyr641)抗体只能识别在tyrosine 641位点磷酸化的内源水平Stat6。抗体不与对应位点的tyrosine磷酸化的Stat蛋白交叉反应。此多克隆抗体是通过合成对应人源性Stat6 Tyr641位点周围的肽段免疫动物而获得。 抗体是通过protein A和多肽亲和层析纯化。受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活，这对IL-4 和 IL-13的应答是必需的(1-4)。另外， 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.