PhosphoPlus? Stat5 (Tyr694) Antibody Kit

• 产品编号：CST-9350S      品牌：CST       原厂货号：9350S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
• 应用分类：

其它组分：

描述：

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 647 Conjugate)检测内源在Tyr694位点磷酸化的Stat5a蛋白和在Tyr699位点磷酸化的Stat5b蛋白。抗体不与对应位点的tyrosine残基磷酸化的Stat蛋白交叉反应，但是与EGFR交叉反应。Stat5 抗体能检测内源总体水平的 Stat5α 和 Stat5β蛋白。此多克隆抗体是通过合成对应鼠源性Stat5a Tyr694 位点周围的磷肽免疫动物而获得(Phospho-Stat5 (Tyr694) Antibody)，或者是通过合成Stat5对应的N-末端区域的多肽免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。PhosphoPlus® Stat5 (Tyr694) Antibody Kit 为Tyr694位点磷酸化的Stat5a和Tyr699位点磷酸化的Stat5b的研究提供了试剂和步骤指导。Stat5的激活是通过一系列的配体的结合而完成，包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5，这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

原厂资料：

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) Antibody detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. This antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins, but does cross-react with EGFR. Stat5 Antibody detects endogenous levels of total Stat5a and Stat5b.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr694 of mouse Stat5a (Phospho-Stat5 (Tyr694) Antibody) or a synthetic peptide corresponding to an amino-terminal region of Stat5. Antibodies were purified by protein A and peptide affinity chromatography.

Description

The PhosphoPlus® Stat5 (Tyr694) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Stat5a at Tyr694 and Stat5b at Tyr699.

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies