RSK3 Antibody

• 产品编号：CST-9343S      品牌：CST       原厂货号：9343S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RSK3 Antibody兔多抗能检测RSK3蛋白水平。该抗体不能与RSK1 、 RSK2异构体发生交叉反应。该多克隆抗体通过使用与人源RSK3蛋白Pro709位点周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族，其特征是包含2个不同的功能性激酶结构域（1）和一个羧基端的细胞外信号调节激酶（ERKs）结合位点（2）。RSK激酶结构域内或以外的一些位点，包括Ser380, Thr359, Ser363和Thr573，对于激酶的激活非常重要（3）。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下，通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活（3）。与他RSK亚型不同，RSK3的N端区域包含一个共有的细胞核定位信号，在血清饥饿模型的HeLa细胞中，该激酶能转位到胞核处(4)。RSK3在人类组织中广泛表达，在小鼠中定位于发育性神经和感觉区域内(5)。相关基因的突变和表达水平的变化与乳腺癌以及一些癌细胞系有关，暗示该激酶作为一种肿瘤抑制子的重要作用 (6)。

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Zhao, Y. et al. (1995) Mol Cell Biol 15, 4353-63.
5. Zeniou, M. et al. (2002) Hum Mol Genet 11, 2929-40.
6. Bignone, P.A. et al. (2007) Oncogene 26, 683-700.

原厂资料：

Specificity / Sensitivity

RSK3 Antibody detects transfected levels of RSK3 protein. It does not cross-react with the RSK1 or RSK2 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro709 of human RSK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).Unlike other RSK isoforms, the N-terminal region of RSK3 contains a putative nuclear localization signal and the kinase translocates to the nucleus in serum-starved HeLa cells (4). RSK3 is expressed ubiquitously in human tissues and localizes to developing neural and sensory areas in the mouse (5). Altered expression and mutations in the corresponding gene are associated with breast cancer and in some cancer cell lines, suggesting a possible role for this kinase as a tumor suppressor (6).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Zhao, Y. et al. (1995) Mol Cell Biol 15, 4353-63.
5. Zeniou, M. et al. (2002) Hum Mol Genet 11, 2929-40.
6. Bignone, P.A. et al. (2007) Oncogene 26, 683-700.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibod