RSK2 Antibody

• 产品编号：CST-9340S      品牌：CST       原厂货号：9340S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RSK2 Antibody兔多抗能检测内源性总RSK2蛋白水平。该抗体不能与RSK1 、 RSK3异构体发生交叉反应。该多克隆抗体通过使用与人源RSK2蛋白Glu719位点周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族，其特征是包含2个不同的功能性激酶结构域（1）和一个羧基端的细胞外信号调节激酶（ERKs）结合位点（2）。RSK激酶结构域内或以外的一些位点，包括Ser380, Thr359, Ser363和Thr573，对于激酶的激活非常重要（3）。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下，通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活（3）。多种生长因子的刺激会引起RSK2的活化，RSK2是一些通路中重要的下游效应子激酶。在体内，在EGF的刺激下，RSK2能够使CREB的Ser133位点及组蛋白H3磷酸化(4,5)。RSK2对p53的磷酸化作用可能有助于调节染色质结构和细胞周期(6)。RSK2主要表达于大脑，并且对认知功能和学习十分重要。在发育期间，RSK2调节造骨细胞和骨架肌细胞的分化(7,8)。相关基因的突变与Coffin-Lowry syndrome (CLS)有关，CLS是是以智能缺陷和特征性面部异常为特点的伴X染色体的紊乱(9)。

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. De Cesare, D. et al. (1998) Proc Natl Acad Sci USA 95, 12202-7.
5. Sassone-Corsi, P. et al. (1999) Science 285, 886-91.
6. Cho, Y.Y. et al. (2005) Cancer Res 65, 3596-603.
7. Yang, X. et al. (2004) Cell 117, 387-98.
8. Cho, Y.Y. et al. (2007) J Biol Chem 282, 8380-92.
9. Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

RSK2 Antibody detects endogenous levels of total RSK2 protein. It does not cross-react with the RSK1 or RSK3 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu719 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).Stimulation by various growth factors leads to activation of RSK2, which is a critical downstream effector kinase in several pathways. EGF stimulation leads to phosphorylation of CREB at Ser133 and phosphorylation of histone H3 in vivo by RSK2 (4,5). RSK2 phosphorylation of p53 may help regulate chromatin structure and cell cycle (6). RSK2 is prominently expressed in the brain and is essential for cognitive function and learning. During development, RSK2 regulates the differentiation of osteoblasts and skeletal muscle cells (7,8). Mutations in the corresponding gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (9).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. De Cesare, D. et al. (1998) Proc Natl Acad Sci USA 95, 12202-7.
5. Sassone-Corsi, P. et al. (1999) Science 285, 886-91.
6. Cho, Y.Y. et al. (2005) Cancer Res 65, 3596-603.
7. Yang, X. et al. (2004) Cell 117, 387-98.
8. Cho, Y.Y. et al. (2007) J Biol Chem 282, 8380-92.
9. Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody