GSK-3α Antibody

• 产品编号：CST-9338S      品牌：CST       原厂货号：9338S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GSK-3a Antibody可以检测内源性总GSK-3α蛋白。其不和重组的GSK-3β发生交叉反应 。多克隆抗体是采用合成的与人源GSK-3α蛋白序列相对应的肽段免疫动物生产的，抗体由protein A和肽亲和层析法纯化。糖原合酶激酶-3（GSK-3）最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶（1）。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子，其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制（2,3）。GSK-3不仅参与盘基网柄菌细胞命运的调控，而且是果蝇，爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分（4）。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位（5）。GSK-3a 调节β淀粉样肽的合成，后者是在阿尔兹海默症发展过程中积累的斑块的主要成分。给予治疗剂量的锂--一种GSK-3抑制剂，可以减少β淀粉样肽的产生，因此阻断了含有过量APP的小鼠大脑中淀粉样-β肽的积累。而这是通过特异性的抑制由γ-分泌酶介导的淀粉样前体蛋白（APP）的剪切来实现的（6）。

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Phiel, C.J. et al. (2003) Nature 423, 435-9.

原厂资料：

Specificity / Sensitivity

GSK-3alpha Antibody detects endogenous levels of total GSK-3α protein. It does not cross-react with recombinant GSK-3β.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3α. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3alpha regulates the production of amyloid-beta peptides, a major component of the plaques that accumulate with progression of Alzheimer's disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-beta production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by gamma secretase, blocking accumulation of amyloid-beta peptides in the brains of mice that overproduce APP (6).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Phiel, C.J. et al. (2003) Nature 423, 435-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody